How do you use isoflurane for anesthesia?

Allow some space on either side. Slightly moisten the end of a small piece of gauze with the Isoflurane mixture. Insert the gauze into syringe nose cone of appropriate size, with moistened end away from open end of the nose cone. Place prepared nose cone into anesthesia jar, until ready to use.

What is an anesthesia chamber?

Anesthesia Chambers allow a method of anesthetic induction without use of short-acting barbiturates. Removable dividers accommodate various size animals – eliminating wasted gas and saving money. Closed airtight system creates a healthy atmosphere for working staff.

What volume of oxygen flow and concentration of anesthetic isoflurane are appropriate for a chamber induction?

Oxygen flow to the imaging chamber and injection station should be set to a maximum of 0.5 lpm, and the induction chamber to a maximum of 2 lpm.

What type of anesthesia is isoflurane?

Isoflurane is a general inhalation anesthetic used for induction and maintenance of general anesthesia. It induces muscle relaxation and reduces pains sensitivity by altering tissue excitability.

How do you dilute isoflurane?

Dilute Isoflurane in light paraffin oil (not mineral oil, available from CRS) at a concentration of 15% v/v (e.g. 15 cc anesthetic in 85 cc light paraffin oil). Shake vigorously to produce even suspension. Saturate cotton or gauze in bottom of small induction chamber with tight fitting lid.

What is an induction chamber?

Induction chambers offer an easy method for anesthetizing small animals and rodents in a research laboratory. These units are designed with sure seal lids and may be connected directly to the bio-evacuation system to prevent laboratory personnel from unnecessary exposure to the anesthesia.

How do you calculate isoflurane?

Barletta. “That’s the partial pressure we need to reach in the brain. In order to have 99% of animals not move, we need to multiply the MAC by 1.4 or 1.5.” Isoflurane in dogs, for example, would be calculated like this: 1.4% x 1.5 = 2.1%.

How do you measure isoflurane levels?

2) Visually check the level of Isoflurane in the vaporizer. It should be at the half way mark to ensure that you do not run out of Isoflurane in the middle of a surgery. a) If the Isoflurane level is below the half way mark then additional Isoflurane is stored in the bottom of the cabinet in the treatment room.

What schedule drug is isoflurane?

Because isoflurane is a Schedule VI drug, all PIs who store and use isoflurane as a part of their research must have a Virginia Board of Pharmacy controlled substances registration.

Why is isoflurane used in cardiac surgery?

Although arterial and systemic blood pressure decline with the use of isoflurane, cardiac output is preserved as the result of an active carotid baroreceptor reflex and decreased afterload. In fact, in contrast to other volatile agents isoflurane may result in the greatest decrease in systemic vascular resistance.

What is open drop anesthesia?

A technique for producing inhalation anesthesia in which drops of a liquid anesthetic, such as ether, are placed on a gauze mask or cone applied over the mouth and nose.

What is isoflurane?

Isoflurane is a volatile anesthetic approved by the Federal Drug Administration (FDA) for the induction and maintenance of general anesthesia. Like most volatile anesthetics, isoflurane is a halogenated ether compound that is a structural isomer to its predecessor, enflurane and has been approved for use in the United States since 1979.

How will EHS evaluate staff exposures to isoflurane?

Upon request, EHS will evaluate staff exposures by monitoring laboratory workers while they perform work with isoflurane. NIOSH has a non-regulatory recommended exposure limit (REL) for halogenated agents (e.g., isoflurane) of 2 ppm or 15 mg/m3 as a ceiling limit (over a sampling period not to exceed one hour) during anesthetic administration.

What is the resting state of isoflurane under normal atmospheric pressure?

In the case of isoflurane, which has a vapor pressure of 240 mm Hg, at a normal atmospheric pressure of 760 mm Hg, the resting state is in its liquid form.  If the atmospheric pressure were decreased to 240 mm Hg, the resting state of isoflurane would be as a gas.

Why choose Kent Scientific for isoflurane vaporization?

For your safety, the safety of your animals and the integrity of your study, Kent Scientific offers genuine, new vaporizers manufactured specifically for either isoflurane or sevoflurane.