How is uric acid synthesized?

Uric acid is synthesized mainly in the liver, intestines and the vascular endothelium as the end product of an exogenous pool of purines, and endogenously from damaged, dying and dead cells, whereby nucleic acids, adenine and guanine, are degraded into uric acid.

What causes gout PDF?

With gout, excess uric acid builds up in the joints and forms needle-like crystals that cause extreme pain. A gout attack can also be caused by joint injury, infec- tion, kidney problems, medications or diet habits (e.g., a purine-rich, sugary diet, drinking too much alcohol or not enough water).

What is the principle of uric acid?

PRINCIPLE (1) (3) Uricase acts on uric acid to produce allantoin, carbon dioxide and hydrogen peroxide. Hydrogen peroxide in the presence of peroxidase reacts with a chromogen (amino-antipyrine and dichloro- hydroxybenzen sulfonate) to yeld quinoneimine, a red coloured complex.

Which method is most commonly used for uric acid estimation?

Currently, two methods are widely utilized to quantify uric acid. A colorimetric method depends on the reduction of a chromogen such as sodium tungstate by uric acid to produce a measurable color change. This technique has been commonly employed in automated hospital screening (SMA systems).

What is the origin and fate of uric acid?

Uric acid is a chemical created when the body breaks down substances called purines. Purines are normally produced in the body and are also found in some foods and drinks. Foods with high content of purines include liver, anchovies, mackerel, dried beans and peas, and beer.

What is converted to uric acid?

Uric acid is generated from metabolic conversion of either exogenous (dietary) or endogenous purines, primarily in the liver and intestine. The immediate precursor of uric acid is xanthine, which is metabolized to uric acid by either xanthine oxidase or by its isoform, xanthine dehydrogenase.

What is gout Slideshare?

DEFINATION  Gout is a metabolic disorder characterized by hyperuricemia which occurs due to defective purine metabolism.  Hyperuricemia can lead to deposition of sodium urate crystals in the tissues especially in joints and kidney.

What is the clinical significance of uric acid?

Uric acid is a useful diagnostic tool as screening for most of purine metabolic disorders. The importance of uric acid measurement in plasma and urine with respect of metabolic disorders is highlighted. Not only gout and renal stones are indications to send blood to the laboratory for uric acid examination.

How is uric acid reagent prepared?

Uric Acid reagent: 4-AAP >0.2mM, HDCBS 2mM, Uricase (Microbial) >150 U/L, Peroxidase (horseradish) >2,500 U/L, Buffer, pH 8.1 ± 0.1, Non-reactive stabilizers. The reagent is ready to use. The reagent set is stored at 2-8°C. Under proper storage the reagent will remain stable until the indicated expiration date.

How is uric acid measured?

A uric acid test can be done as a blood test or a urine test. During a blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial.

What are the clinical significance of uric acid?