What is CD CHO?
CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture.
What is in CD CHO media?
CD CHO is an animal origin-free, chemically defined medium that contains no proteins, hydrolysates, or components of unknown composition. For research use/further cell culture manufacturing.
What is Cho science?
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins.
What is a CHO screening?
Abstract. Early analytical clone screening is important during Chinese hamster ovary (CHO) cell line development of biotherapeutic proteins to select a clonally derived cell line with most favorable stability and product quality.
Is CHO an immortalized cell line?
Chinese hamster ovary (CHO) cells are a source of multi-ton quantities of protein pharmaceuticals. They are, however, immortalized cells, characterized by a high degree of genetic and phenotypic diversity.
What are CHO cell lines used for?
CHO cells, which are a cell line derived from the ovary of the Chinese hamster, meant for use in biological and medical research and commercially in the production of therapeutic proteins.
Are CHO cells immortal?
Fact #3 – CHO cells are not immortal Well, while they are tiny yet tough, they are not “immortal”. Much rather, they are what is called “immortalized”.
Are CHO cells cancerous?
CHO cells can also be kept in suspension cultures; in contrast to cancer cells, they are genetically stable; they can be reproduced with expression vectors that contain the “gene of interest” (GOI); they can be transfected; and they remain stable during the process of selection, amplification, single-cell cloning and …
How fast do CHO cells grow?
between 14 and 17 hours
For one, CHO cells make it easy to scale up production: They have a doubling time of between 14 and 17 hours. Growth densities exceed 5 million cells per 1 milliliter in proper conditions.
Are CHO cells immortalized?
Can CHO cells be growing in suspension?
Suspension CHO cells grown in most modern media formulations, such as CHOgro® Expression Medium from Mirus Bio, can easily reach densities of 1-2×107 cells/ml. The doubling time is typically 24 hours for contact-inhibited adherent CHO-K1 cultures but can decrease to nearly half that for suspension CHO cells.
How are CHO cells harvested?
The conventional method of harvesting consists of a centrifugation step in which particles (cells, debris, etc.) with high density or large size are separated.