What color is Feulgen stain?

magenta colour
The Feulgen reaction is based on Schiff’s reaction for aldehydes whereby, by acid hydrolysis, the liberated aldehydes of the deoxy sugar are allowed to react with fuchsin–sulphurous acid to yield a typical magenta colour reaction.

What is Feulgen stain used for?

The Feulgen technique selectively stains DNA, and under controlled conditions, can be used for the photometric determination of DNA content.

What type of stain is Feulgen stain?

Feulgen stain is a staining technique discovered by Robert Feulgen and used in histology to identify chromosomal material or DNA in cell specimens. It is darkly stained. It depends on acid hydrolysis of DNA, therefore fixating agents using strong acids should be avoided.

What is Feulgen positive and negative?

SUMMARY. The nuclei of the male and female gametocytes of the malarial parasites {Plasmo- dium and Hepatocystis) are Feulgen-negative, while the nuclei of the gametocytes of Hepatozoon, which are not sexually differentiated, are Feulgen-positive.

Does Feulgen stain RNA?

RNA, where the purine linkage is acid-stable, did not react. Feulgen staining enabled nuclei to be clearly identified in animal and plant cells. The next step was to find a quantitative method for the estimation of the DNA in the nucleus. A suitable method for this was introduced by Caspersson (mid-1920s–ca.

What did Feulgen discover?

Joachim Wilhelm Robert Feulgen (2 September 1884 – 24 October 1955) was a German physician and chemist who, in 1914, developed a method for staining DNA (now known as the Feulgen stain) and who also discovered plant and animal nuclear DNA (“thymonucleic acid”) congeniality.

Does feulgen stain RNA?

Who have developed Feulgen technique?

Abstract. The Feulgen reaction proposed by Feulgen and Rossenbeck 75 years ago is one of the cytohistochemical reactions most widely used in biology and medicine.

What reaction to Feulgen stain is made by DNA?

The principle of Feulgen stain is to dissociate the two strands of DNA through hydrolysis by a solution of molar HCl which destroys the purine bases. HCl separates the two purine bases of DNA: adenine, guanine, liberating the hemiacetal functions of deoxyriboses.

How Feulgen stain is prepared?

In a routine Feulgen staining technique, slides are immersed in 5 mol/L HCl for 15 minutes, rinsed with distilled water for 3 minutes, stained with Schiff’s reagent for 90 minutes, washed for 10 minutes and finally stained with 1% light green for 15 minutes (7-8).

How feulgen stain is prepared?

Is feulgen stain a special stain?

Feulgen stain can visualize DNA on tissue sections and in cells. This staining is the most used staining to highlight DNA in histology. The principle of Feulgen stain is to dissociate the two strands of DNA through hydrolysis by a solution of molar HCl which destroys the purine bases.

What is Feulgen stain in histology?

Feulgen stain. Feulgen stain is a staining technique discovered by Robert Feulgen and used in histology to identify chromosomal material or DNA in cell specimens.It is darkly stained. It depends on acid hydrolysis of DNA, therefore fixating agents using strong acids should be avoided. The specimen is subjected to warm (60 °C)…

What is the critical preparation of materials through Feulgen staining?

The critical preparation of materials through Feulgen staining depends on several factors, especially hydrolysis, method of fixation and type of tissue used. In addition, the concentration of DNA is no doubt one of the principal factors.

What is the background color of Feulgen reaction?

The background, if counterstained, is green. The Feulgen reaction is a semi-quantitative technique. If the only aldehydes remaining in the cell are those produced from the hydrolysis of DNA, then the technique is quantitative for DNA.

How do you measure Feulgen staining?

Feulgen stain. If the only aldehydes remaining in the cell are those produced from the hydrolysis of DNA, then the technique is quantitative for DNA. It is possible to use an instrument known as a microdensitometer or microspectrophotometer to actually measure the intensity of the pink Feulgen reaction for a given organelle.